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This combination of read length and paired reads spanning quite large distances can be used effectively by assembly algorithms to resolve many repeats and reconstruct a draft genome sequence (Myers et al. 2000; Batzoglou et al. 2002; Jaffe et al. 2003).
No predicted miRNA had the ideal combination of read mapping signature and secondary structure to be considered a confident miRNA.
For each tool we show the combination of read length and layout that maximised the ROC performance.
It can be used on any combination of read datasets and can be easily adapted to other technologies arising in the future.
Capillary-based sequencing remained cost prohibitive, but until recently, newer technologies did not offer the combination of read length and insert size needed to de novo assemble large genomes.
When considering each combination of read layout, length and fusion finding algorithm, we found that JAFFA with 100 bp paired-end reads produced the highest number of true positives, with a total of 27.
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"You see other players in the league being able to skate," Brodeur said, "but it was the combination of reading the play, making the right decision and having the mobility that made Scott so special".
The students — sixth-, seventh- and eighth-graders — spend half the day with Rawson and Hang, learning a combination of reading comprehension, language skills and the basics of how to go to school, like taking notes. .
The combination of reads derived from a single set of transcriptomic data (30 million reads) enables comprehensive examination of ∼3000 of the gene models in the Bestmodelv1 set using a transcript sequence coverage depth of 23.
We tried to assemble contigs from different combination of reads.
A combination of reads showing variation and read depth, along with mapping quality and SNP quality were considered for filtering the SNPs (Additional file 12).
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CEO of Professional Science Editing for Scientists @ prosciediting.com