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Combination index.
Synergistic interactions in vitro were evaluated by combination index analysis.
The combination index (CI) was calculated according to the -isobologram equation: CI = D1/Dx1 + D2/Dx2.
b Combination index for MTX-PEG-CPT NRs against HeLa cells.
One way to express the interaction is through a combination index τ based on Loewe additivity.
Statistical analysis: Combination Index (C.I).
The combination index of each treatment was calculated according to the classic isobologram equation combination index = [(D 1/(Dx)1]+[(D 2/(Dx)2] as previously described [52].
Combination index-isobologram equation allowed quantitative determination of drug interactions, where combination index <1, = 1, or >1 indicated synergism, additive effect, or antagonism, respectively.
Combination treatment II had synergistic effect at IC 50, 70 with combination index of 0.23, 0.121 and additive effect at IC 30 with combination index of 1.025.
A combination index >1 indicates antagonism, = 1 indicates additivity, and <1 indicates synergy.
The tumor doubling time (TDT) and combination index (CI) were calculated using GraphPad Prism v4.0.
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