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We report the efficacy of this combination in cell lines from many different cancers.
Thus, the cytotoxic response observed with this drug combination in cell proliferation assays is the result of an increase in apoptosis that is not seen with either agent alone.
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In the comparative study presented here, we aimed to characterize different commonly used small epitope tag/antibody combinations in cell culture as well as in vivo.
Beyond nomination of effective drug combinations in cell lines, we suggest that the perturbation biology method more generally paves the way for model-driven quantitative cell biology with diverse applications in many fields of biology.
Finally, we validated the association of miR-564 targets with cell invasiveness using 58 cancer cell lines in NCI60 panel and 12 breast cancer cell lines from GSE40059 dataset where higher levels of target genes expression in combination in invasive cell lines compared to less invasive cell lines were observed (Fig. 4G,H).
The process of cell invasion is a combination of in cell migration with concurrent degradation of the surrounding ECM.
To the best of our knowledge, it is the novel signalling combination identified in cell adhesion and spreading process in human gastric carcinoma.
This enhanced target response is consistent with the improved growth inhibition that we observed using the drug combinations in cell-based assays (Fig. 2C,D).
Finally, we tested this combination in different cell types and compared primary human fibroblasts (mesenchymal cells) with primary human keratinocytes (epithelial cells) and immortalized human microvascular endothelial cells.
Genistein potentiated growth inhibition and apoptosis of the gemcitabine and erlotinib combination in COLO-357 celineine.
This means that there is no significative impact of service combination in the cell radius.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com