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Combining DGE with an RNA-sequencing generated reference transcriptome provides an excellent combination for analysis of non model plant species.
To assess the effect of multi-region combinations on species resolution, we selected a subset of the possible two-, three-, four-, five- and six-region combinations, in addition to the seven-region combination for analysis (Table S2) (cox1 and 23S rDNA were excluded because of their low individual success, see Results).
The four unique sequence reads per template are accurately traceable during sequence alignment and can be used separately or in combination for analysis.
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Requiring more than one SNP per gene reduces the number of gene x tissue combinations for analysis to 4,667 and also reduces the number of genes displaying major allelic ratios greater than 5-fold (<1%).
Gas chromatography (GC) and mass spectrometry (MS) has proved an effective combination for chemical analysis, in order to carry out simultaneous identification and quantification of the various components of complex mixture [9].
Further research aimed at finding other sensitive biological markers in PE and for defining the best combination for marker analysis is warranted as well.
Consistently, the hyphenation of UHPLC to linear ion trap Orbitrap MS represents a highly promising combination for UHPLC MS analysis of carotenoids.
Although our results are of significant merit in both the clinical and pathogenetic aspects of lung cancer, further research aimed at defining the best combination for marker analysis is warranted.
The mean of the s.e. of the QTL effects in the selected model was calculated for each combination of analysis method, trait, significance threshold, replicate and fold.
This task works with a combination of analysis and processing for movie frames, shots, scenes, segmentation and textual description.
Three replicated plants from each treatment combination were selected for analysis.
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