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The configuration of both, traditional and dividing wall reactive distillation columns, were obtained from the conceptual analysis.
The column strengths, failure modes and load-axial shortening curves of the columns were obtained from the FEA.
DEAE-Sepharose Fast Flow and Sephadex G-25 columns were obtained from Pharmacia (Uppsala, Sweden).
DEAE-Sepharose, HiLoad 16/60 SuperdexTM 200 prep grade column, and PD-10 desalting columns were obtained from GE Healthcare Bio-Sciences CorPiscatawayaway, NJ, USA).
RP-HPLC columns were obtained from Phenomenex (Torrance, California, USA).
OchraTest WB columns were obtained from Vicam (Watertown, MA, USA).
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where K is a R×Q matrix whose columns are obtained from B as: {fontsize{8.7pt}{9.6pt}selectfont{begin{aligned} {mathbf{k}}_{q}!
Glass wool to perform columns was obtained from Micro-Fibre Manville (Denver, CO, USA).
BCA reagent for protein assay and protein A column were obtained from Pierce Biotechnology Inc. (Rockford, IL, USA).
Strata-X 200 ml/3 ml, Strata-X-AW 200 ml/3 ml, Strata CN 500 ml/3 ml, and Strata Phenyl 500 ml/3 ml SPE-columns were obtained from Phenomenex (Torrance, CA, USA) and applied in optimizing the SPE procedure.
M13KO7 helper phage, HRP-anti-E tag conjugate and an anti-E-tag Sepharose column were obtained from GE Healthcare UK Ltd., UK.
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