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Associations between current smoking and categorical variables forming tables with two rows and two columns were assessed using the Yates corrected Chi-square test, and the Pearson Chi-square test was used for higher contingency tables.
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In addition, the current design rules in the North American Specification for cold-formed steel non-symmetric lipped angle columns are assessed using reliability analysis.
Protein concentrations were assessed using the Pierce ™ BCA Protein Assay Kit
Time trends were assessed using birth cohorts.
Potential interactions were assessed using logistic regression.
Differences between groups were assessed using ANOVA.
Healthy practitioners of meditative mindfulness and a control group were assessed using a row/column speller task [ 42].
Percentage yields for chemical reactions as described are quoted only for those compounds that were purified by recrystallization or by column chromatography, and purity was assessed using TLC or H NMR spectroscopy.
RNA was purified using Micro RNEasy columns (Qiagen) and RNA quality was assessed using Agilent Bioanalyzer.
Primer sequences used are shown in Table 2.> Total RNA was purified with RNeasy Minikit columns (Qiagen) and RNA quality was assessed using RNA 6000 Nano chips on the Agilent 2100 Bioanalyzer (Agilent Technologies, Amsterdam, The Netherlands).
Microbial activity for the root zone, water column, and gravel and sludge was assessed using the fluorescein diacetate assay (FDA) method (Adam and Duncan 2001; Sirage 2012).
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