The purification of the compounds was carried out on Agilent 1100 HPLC system equipped with a quaternary pump, a diode array detector and a reverse phase silica gel column (Phenomenex Synergy Hydro-RP, 250 mm × 100 mm, 4 μm) or normal phase silica gel column (Sphereclone silica 250 × 10 mm column) at a flow rate of 1.0 mL/min.
The column was a Synergy max RP (150 mm × 3.0 mm) with a particle size of 4 μm (Phenomenex) maintained at 27°C.
An aliquot of the extract was used for analysis by HPLC configured with LC-10AD pumps, SLC10A system control, diode array UV-VIS detector (Shimadzu Japan) and Synergy Hydro column (4 μm, 250 mm × 4.6 mm; Phenomenex).
The column used was a Phenomenex Synergy Hydro-RP 80A (Torrance, CA, USA) (150 × 3 mm, 4 μm).
After automated backflush elution, chromatographic separation was performed on a Synergy Fusion-RP column (250 × 2.0 mm, 4 μm, Phenomenex, Germany).
McCartney's dynamic image and gossip column friendly lifestyle create a natural synergy with the glamorous Milanese company.
Separation was performed with Synergy fusion-RP analytical column (250 mm × 2.0 mm, 4 μm particle diameter; Phenomenex, Torrance, CA, USA) preceded by a guard column (4 mm × 2 mm) of the same packing material from Phenomenex.
In order to predict the performance from startup to stable operation in CO2 absorption process, a multi-field synergy model was developed based on CO2 capture process in a packed column by means of monoethanolamine (MEA).
DNA was purified with MinElute columns (Qiagen) and quantified by fluorimetry with PicoGreen (Molecular Probes, Eugene, OR) on a Synergy HT plate reader (Biotek, Winooski,VT) or by NanoDrop spectrophotometer (ThermoScientific, Fisher).
In addition, analytic hierarchy process (AHP) is used to determine the intensity of the relationship between the row and column variables of each matrix, while analytic network process (ANP) is used to determine the intensity of synergy effects among column variables.
The qualitative analysis of the residual soluble oligosaccharides or polysaccharides from Raftilose Synergy, Raftiline HP and Raftilose P95 was carried out by HPAEC-PAD, using a CarboPac PA100 column and an appositely optimized gradient elution program.
