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Exact(31)

The overall quality of these automatic alignments was measured using the Column Score (CS) described in Methods.

The ability of the programs to align a specific block was estimated by calculating the block column score

The alignment quality for each individual block was then measured using the Block Column Score (BCS) described in Methods. Figure 6(A D) shows the distributions of the block scores obtained by each MSA program for each block attribute.

We calculated two scores to quantify this accuracy (Figure 2 A,B): The column score (CS) is the proportion of columns from the true alignment that are present and correct in the test alignment.

A local alignment requires that the expected column score be negative and some column score(s) be positive.

Equation (2) is based on the column score derived in [ 33].

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Similar(29)

A score for each column in the alignment is calculated using the concept of continuous sequence space introduced by Vingron and Sibbald [64] and the column scores are then summed over the full length of the alignment.

Average Column scores are given for each method.

The score of the final alignment is then summed over all column scores.

Each of the column scores can then be converted to a T-score.

Once all column scores have been computed trimAl can proceed in two ways.

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