Semipreparative HPLC was performed on a Waters 1525EF liquid chromatography system equipped with a Hypersil BDS C18 column (4.6 mm × 250 mm; 10.0 mm × 250 mm) and a Chiral column Chiralpak AD-H (4.6 mm × 250 mm).
50 ml of the hydrolyzed-DNA solution were injected onto an Atlantis dC18 column (2.1×150 mm; 5 mm particle size) protected by an Agilent guard column (2.1×20 mm; 5 mm particle size) at a constant flow of 0.220 ml min−1.
The analytical CarboPac PA-100 column (250 mm × 4 mm) and the guard column PA-100 (25 mm × 3 mm) (Dionex) were used for disaccharide and oligosaccharide analysis.
Oligocellulose mixtures were analyzed by IC on the Dionex ICS2500 system with a CarboPac PA100 guard column (4 mm × 50 mm), a CarboPac PA100 analytical column (4 mm × 250 mm) and an ED50A integrated amperometry detector (Dionex, Sunnyvale, CA, USA).
The predicted gradient profile was further transferred to a long UHPLC column (2.1 mm × 100 mm, 1.7 μm) and a conventional HPLC columns (2.1 mm × 100 mm, 3.5 μm and 4 mm × 100 mm, 5 μm, respectively).
The HPAEC system ICS-50000, Dionex, Sunnyvale, CA, USA) was equipped with a combination of a CarboPac PA1 guard column (50 mm × 2 mm i.d., Dionex) and a CarboPac PA1 analytical column (250 mm × 2 mm i.d., Dionex).
Sugars were separated on a Dionex Carbo Pac PA 10 analytical column (250 mm × 4 mm, 10 μm) connected to a Carbo Pac PA 10 guard column (50 mm × 4 mm).
We used an Alltech (Queensland, Australia) Prevail C18 column (150 mm × 4.6 mm, 5 μm) with a Phenomenex (California, USA) Security C18 guard column (2 mm × 4 mm, 5 μm).
Chromatographic separation of peptides was carried out on an Ultrasphere ODS column (250 mm × 4.6 mm; 5 µm) following a pre-column (45 × 4.6 mm I.D).
Chromatographic separation was performed on a Halo Amide reversed C18 column (50 mm × 2.1 mm, i.d. 2.7 μm, 92812-407, Advanced Materials Technology) protected by a Halo Amide guard column (5 mm × 2.1 mm, i.d. 2.7 μm, 92812-107).
