Sentence examples for column equilibration from inspiring English sources

Another practical advantage is a short time needed for column equilibration when a mobile phase gradient is used [29].

Binding buffer (100 mM Tris HCl, 500 mM NaCl, 20 mM imidazole, pH 7.5) was used for cells resuspension and His SpinTrap™ column equilibration.

Triethylamine should be avoided as its addition to the mobile phase requires longer column equilibration times, which is not advisable for the routine use of the developed method.

Mobile phase A (Millipore H2O) and mobile phase B (acetonitrile) in gradients: 0 5 min; 15% B in A (isocratic run), 5 27 min; 15 100% B (gradient mode), 27 32 min; 15% B in A (for column equilibration).

The automated 2-step (cation exchange and size exclusion) purification protocol for untagged hCypA results in final purity and yields of ⩾93% and ∼5 mg L−1 of original cell culture, respectively, in under 12 h, including all primary sample processing and column equilibration steps.

The novel automated 4-step (anion exchange, desalt, heparin-affinity and size exclusion, in linear sequence) purification protocol for untagged hPCNA results in final purity and yields of ⩾87% and ∼4 mg L−1 of original cell culture, respectively, in under 24 h, including all primary sample processing and column equilibration steps.

Samples were subjected to spin-column equilibration using Bio-Spin Columns with Bio-Gel P-30 (Bio-Rad).

A volume of 100 µl at a concentration of 24 µM was loaded into the column after equilibration with the corresponding buffers (at 50 mM), at the suitable pH, and with 150 mM NaCl.

For the latter step, the protein solution was applied to the column after equilibration with 50 m m Tris HCl buffer, pH 8.0, containing 100 m m NaCl.

In the first step of purification the lysate was loaded onto a 75 ml bed volume phosphocellulose cation exchanger column pre-equilibrated with equilibration buffer (50 mM PIPES, pH 7.0, 100 mM NaCl, 1 mM EGTA and 1× protease inhibitor cocktail).

Briefly, the cell supernatant was loaded onto a 3-ml nickel-nitrilotriacetic acid-agarose resin (Ni-NTA agarose, Cat. No. 30210, Qiagen, Hilden, Germany) packed column pre-equilibrated with equilibration buffer (25 mM Tris-Cl pH 8.0, 300 mM NaCl).