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Height of CSF column (cm) was plotted against time and the best linear fit determined for each data set.
The dialyzed sample was centrifuged at 27,000 × g for 30 min at 4°C, and the supernatant was applied to a carboxymethyl (CM -cellulose CM -cellulose, Whatman International Ltd., Maidstone, UK) (3.4 × 30 columnuilibrated with 0.01 M MES buffer (pH 6.0).
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The concentrated supernatant (2 ml) was subjected to gel filtration using Sephadex75 column (30 cm x 1 cm, Pharmacia) placed in tandem with a Superdex G200 column (30 x 1 cm, Pharmacia) using AKTA FPLC system.
For the experiments performed using the expanded bed, a homemade column (2.6 cm × 30.0 cm) was specially designed.
The diluted fraction (409 mL) was applied to a Q-Sepharose column (2.6 cm × 15 cm).
The products were loaded onto a Sephadex S200 column (1.6 cm × 60 cm).
After digestion, a Sephadex G25 column (2 cm × 80 cm) was employed for separation purpose.
The sample solution (pH 7.0) was applied to a glass column (120.0 cm × 7.5 cm i.d).
Silica gel 60 PF254 was packed into the VLC column (10 cm × 10 cm) and washed with hexane until fully packed.
To quantify phototactic behaviour, we used an experimental glass column (25 cm high, 5 cm internal diameter).
The supernatant was loaded onto a Ni-NTA column (2 cm × 11 cm), pre-equilibrated with lysis buffer.
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