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The images acquired with the different microscope filters for a given field were merged to obtain a single three coloured image, where blue always corresponded to the total cell nuclei that incorporated the Hoechst dye.
They demonstrated that MVQ achieved a time complexity of Nlog2 M, where N is the number of pixels in the image and M the number of the desired clusters in which the coloured image is dithered.
This is graphically represented as a 2-dimensional coloured image, where each entry of the matrix is coloured on the basis of its value, and where the rows and columns are reordered according to the hierarchical clustering.
The coloured image (green) shows the merged stack images of 14 Z-slices, the other images show the individual Z-images (1 μm depth) of HeLa cells expressing GFP-BtpA.
This type of representation is based on a hierarchical clustering simultaneously operating on the rows and columns of a real-valued similarity matrix M. The initial matrix is graphically represented as a 2-dimensional coloured image, where each entry of the matrix is coloured on the basis of its value, and where the rows and columns are reordered according to a hierarchical clustering.
Mix the resulted layer ("grain extract layer") with the original coloured image (from step 3) using the "Multiply function".
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Two differently coloured images have been slid in front of her cage.
Comparison of the results obtained for the different visual scenes suggests that the greatest level of human emotional activation was achieved by the Infrared Blue-Red (thermal image) format, and that, generally, coloured images provided higher levels of emotional activation than gray-scale images.
Fluorescent coloured images were separated into individual colours and converted to binary images, manually thresholded and segmented using ImageJ (v1.41 http://rsb.info.nih.gov/ij/).
The fusion of the coloured images can be performed by representing images in different colour spaces (such as the RGB, HSV and Lab colour spaces).
Adults (n = 16) matched one of two coloured images with a third stimulus, the target, by either the colour or shape dimension of the target.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com