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This coloured compound, even in low concentration, is highly visible if it is present in the water bodies causing unaesthetic view.
The method is based on the diazotization reaction of dapsone (4,4′-diamino-diphenyl sulphone, DAP) and (naphthyl)ethylenediamine hydrochloride (NED) with nitrite in acidic medium, yielding a coloured compound on the surface of a filter paper.
UV (methanol): λmax = 221 nm (double bonds presence), at 528 nm (coloured compound).
L-lactate concentration is determined by an enzyme assay, based on the L-lactate oxidation by L-lactate dehydrogenase, and the subsequent interaction with a probe which results in the formation of a coloured compound.
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Dyes are coloured compounds suitable for colouring textiles, wool, leather, paper and fibres.
A second class of highly coloured compounds that have distinctive visible absorption are coordination compounds of the transition elements.
These brilliantly coloured compounds have found wide application as dyes and as chemical indicators of acidity or alkalinity.
The solvent is then allowed to evaporate, and the location of the separated components is identified, usually by application of reagents that form coloured compounds with the substances.
Before the flowing solvent reaches the farther edge of the paper, both solvents are evaporated, and the location of the separated components is identified, usually by application of reagents that form coloured compounds with the separated substances.
Covalently bonded molecules consisting of nonmetal atoms and carbon compounds with no aromatic rings or conjugated chains have all their inner orbitals fully occupied with electrons, and for the majority of them the first unoccupied MOs tend to lie at considerably higher energies than in visibly coloured compounds.
Thus, no useful information on the coloured compounds was obtained.
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