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The resulting yellow coloured complex was extracted in chloroform and showed absorption maxima at 340 nm.
Among the fractions, ethyl acetate fraction exhibited highest inhibition of 93.14%, 89.99%, 87.04% in DPPH, non-site specific and site specific deoxyribose degradation assays, respectively and 91.20% reduction of ferricyanide to give Prussian blue coloured complex in reducing power assay at maximum concentration tested.
In Ferric ion reducing power assay, MEFCA showed strong reducing power forming blue or green coloured complex with reduction of Fe3+/ferricyanide complex in to the ferrous form depending on the reducing power of each concentration of the extract.
With starch sizes, the Iodine reagent (Iodine + Potassium Iodide) produces a coloured complex of intense violet blue colour.
2-HP is a mechanism-based inhibitor of CuAOs (Scheme 2)[ 5a] that gives the yellow coloured complex, adduct I.[ 14] A change in solution colour from pink (unreacted TPQ at 480 nm) to yellow (adduct I complex at 420 nm) was only observed with WT/WT and WT/Y382F AGAOs, but not with Y382F/Y382F, as expected.
The indicators are dyes that have the property of forming a coloured complex with the metal ion.
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The polybiguanides readily formed coloured complexes with copper ions.
Maximum absorbance was obtained following heating on a water bath at 80°C for about 10 min with both Fe2+-phen and Fe2+-bipyridyl coloured complexes.
The absorption spectra of the coloured complexes under optimum conditions were scanned in double beam mode against a reagent blank over the range 400 900 nm, and recorded according to general procedures.
The coloured complexes formed were measured at 510 and 521 nm against a reagent blank treated similarly according to methods A and B. Into a series of 10 mL calibrated flasks, aliquots (0.2 1.8 mL) of 100 μg mL-1 standard solutions were transferred using a micro burette and the total volume adjusted to 3 mL by adding bidistilled water.
Deposited calcium phosphates form a purple-coloured complex with o-cresolphthalein complexone in an alkaline medium.
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