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There are also a number of potential extensions to LAMINA that we feel would have broad appeal to leaf researchers, including colour quantification (for example to track senescence), detection of necrotic lesions or flecks, measurement of leaf rust urediospore number and dimensions, and quantification of veinal pattern.
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To the best of our knowledge, this is the first time that the image analysis is reported as useful non-destructive technique to correlate colour and encapsulated materials quantifications.
Another approach is the use of a specifically designed software for quantification of the colour map output [117], achieving area under the ROC curve values of 0.75 for the diagnosis of F ≥ 2, 0.73 for the diagnosis of severe F ≥ 3 and 0.69 for the diagnosis of cirrhosis.
This test format is useful for the detection of proteins, but not for quantification, because the colour transition takes place over a narrow concentration range.
As described previously [57], [59], digital pixel densitometry was performed using Adobe Photoshop CS3 (Adobe Systems Incorporated, USA) utilising the Colour Range tool for colour-based quantification or Lasso tool for manual selection of epithelial mucosa area and subsequent pixel area readout from the Histogram data chart.
The representative images for publication have been adjusted for brightness, contrast and colour balance for ease of viewing, but quantification was performed without any manipulation of the above-mentioned parameters.
After this conversion, a precise and rapid mathematical quantification (in %) of individual colours is possible.
First, the colours of the images were separated, for quantification of vessel density (green) and drug distribution (red).
We present a new method enabling efficient mapping and quantification of large snags by coupling colour infrared aerial photographs and a geographic information system (GIS).
A mean shift segmentation technique gave the best results, but for real-time constraints we simply implemented an image quantification method based on the HSL colour system.
Quantification of Dynamin1 was achieved measuring colour changes using a spectrophotometer at a wavelength of 450nm.
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