Your English writing platform
Discover LudwigExact(1)
Each sample was hybridized with the one colour protocol.
Similar(59)
This method demonstrated very similar performances to the 2-colour protocol [ 97, 127].
bAnti-CD45-PE-Cy7, anti-CD24-PE-Cy5 and the IgG-PE-Cy7 isotype control were only used in the five-colour protocol including CD24 detection.
Flow cytometry data was analysed using FlowJo [ 19]. aAnti-CD45-PE-Cy5 was used for the four-colour protocol not including CD24.
Slides were scanned on the Agilent DNA Microarray Scanner and data extracted using the feature extraction software package (v9.1.3.1) using a standard two-colour protocol.
Our study did not confirm a specific expression for the genes regulated by interferon type I, as described in a RA large-scale expression profiling [8] (two colours analysis protocol using Stanford University microarrays) (data not shown).
The one-colour microarray protocol is described in manual: G4140-90040.
Hybridizations were performed at the Universidad de Santiago de Compostela (USC) Functional Genomics Platform by the Agilent Technology Gene Expression Unit using a 1-colour labeling protocol.
Hybridization, washing and scanning was performed as described in the Two-colour Microarray protocol and the data analysed using GeneSpring GX version 7.3.1.
Hybridization and washing was performed according to the Agilent's two-colour hybridization protocol with the following change: 1 μg of labelled Cy5 and/or Cy3 cRNA was used as input in the hybridization mixture.
cDNA synthesis, labelling and hybridization were done according to the Agilent one-colour microarray protocols (available at http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-color_v6.5.pdf).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com