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The optimal value is 8.20 mL/min for loading flow rate of coloring solution.
For each coloring solution, the total register width is evaluated, and the minimum is taken as the optimal pipeline schedule.
Depending on the top value, the function can return the control, generate the next complete coloring solution and compare it with the best current one, choose the next correct color of the current operator and generate the next record in the stack for procedure recursive call.
The peroxidase activity was visualized with a nickel-enhanced coloring solution (0.2 mg/ml diaminobenzidine: DAB, 0.02% H2O2, 0.03% nickel ammonium in Tris-buffered saline).
The signals of in situ hybridization were detected with a coloring solution consisting of 4.5 µg/ml NBT and 3.5 µg/ml BCIP in 100 mM Tris/HCl (pH 8.0) with 100 mM NaCl and 50 mM MgCl2 (Roche, Germany) in dark for 3 12 h, and then observed and photographed.
Briefly, after seeding the cells onto 96-well plates at the density of 1 × 10 cells/well, the coloring solution was added to the culture media at each time point (0, 1, 2, 3, 4 and 5 days after seeding).
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In this case, a transparent yellow color solution was obtained.
The pale yellow color solution turns to ruby red color indicating the formation of gold nanoparticles.
Nanocomposites have been found partially soluble in DMSO/DMF and give blue color solution.
After that, the color solution in each well was measured at 450/540 nm within 30 min.
The yellow color solution changed to reddish-brown indicating the formation of silver nanoparticles.
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