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Once coloring developed, the absorbance of 200 μl of each sample was analyzed at 490 nm using the Muli-Detection Microplate Reader Synergy2 (Biotek instruments, Witec AG).
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Color developed by the addition of o-phenylenediamine substrate, and color development was stopped by the addition of 4 M H2SO4.
After 5 min the color development was stopped using 2.5 M sulphuric acid and the color developed recorded with a Bio-Rad 680 microplate reader at 450 nm.
The living room is painted in Pantone's Golden Haze, a color developed with the idea of creating sunlight.
The skunks needed to change in order to survive, so new colors developed a.
The mixture was heated in a water bath at 90 °C for 40 min until a pink color developed.
During subsequent acid and acetone rinses in Method 1, no yellow color developed, but modest turbidity was observed.
The merle Pomeranian is a recent color developed by breeders.
The color developed was measured in a SPECTRAmax photometer at 450 nm.
Next, sections were incubated with avidin-biotin-peroxidase complex, the color developed with 3,3'-diaminobenzidine (Vector Laboratories).
If a pink to purple color developed, the reagent was also added to the two drug containing tubes.
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