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But the two compounds were distinguishable by the major molecular ions [M + H2]+ of each coloring compound which are m/z 338 and m/z 354 respectively (Ahn et al., 2014; Ren et al., 2007).
Based on the abundance of the compound in untreated solution, the relative concentration of each coloring compound in the degraded dye solution was calculated in percentage, and the results were plotted in an Excel graph (Figure 4).
HPLC-DAD-MS analysis was initially conducted on the untreated dye solution to find the retention time of the chromatogram peak, UV-visible absorption spectrum (λmax), and major molecular ion of each coloring compound.
The lowering of the peaks suggested decrease in relative abundance of each coloring compound, and visually, such decrease was most prominent in purpurin (4) and indigotin (5) peaks (Figure 3).
The abundance of ion chromatogram was used as the measure of concentration of corresponding coloring compound detected in the HPLC-MS.
The details of the λmax of the UV-visible absorption bands and the major ion detected for each coloring compound follow the analytical results and discussion on the standard dyes reported previously (Ahn et al., 2013; Ahn et al., 2014).
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