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Sensor 1 showed a selective fluorescence enhancement and colorimetric change for Hg2+ in CH3CN HEPES buffer (0.01 M, pH 7.4) (3:7, v/v).
The response is based on a ring opening reaction and formation of a 1 1 complex, while ring-opening process of spirolactam enables large fluorescent enhancement and colorimetric change upon the addition of Hg2+.
Highly selective fluorescent enhancement and distinct colorimetric change were detected upon the addition of bismuth ion (Bi3+) into the sensor solution in EtOH/CH3CN (1:4, v:v) at pH 5.0 7.0.
A highly Hg2+-selective fluorescence enhancing property (>1.5-fold) in conjunction with a visible colorimetric change from yellow to purple has been observed, leading to a potential fabrication of both "naked-eye" and fluorescence detection of Hg2+.
In addition to the electrical signal change in the presence of the target DNA, colorimetric change was also examined after RCA (Fig. 4).
Moreover, the CD solutions simultaneously experience a colorimetric change from dark red to green after adding the H2O2 (inset of the Fig. 1f).
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In addition, colorimetric changes of sensors interacted with Hg2+ are also successfully demonstrated.
Ten microliters of alamarBlue were added and colorimetric changes were measured by Synergy Mx Microplate Reader (BioTek, Winooski, VT, USA) at wavelengths of 570 nm and 600 nm.
Its sensing properties toward various metal cations in aqueous solutions were investigated by colorimetric changes, UV vis and fluorescence spectroscopies.
L exhibited binding-induced colour change from colourless to reddish yellow with a detection limit of 3.66 × 10−7 M, measured by spectroscopic methods, while colorimetric changes could be observed at naked eye for concentrations as low as 2.5 × 10−6 M.
The polymeric sensors are non-fluorescence in the spirolactam form and could be selectively converted into the fluorescence ring-opened amide form in the presence of Au3+, leading to the fluorescence enhancements and colorimetric changes.
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