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Finally, Alexa-700, which has already been used for two color STORM imaging [ 31], blinked in Vectashield even when excited with a 641 nm laser, but the number of detected photons per molecule was almost ten times lower than what is achieved with Alexa-647.
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The company is also debuting two new colors, Storm and Coral.
(B ) Two-color STORM showing abNup358 labeled in blue and impβ labeled in red.
These two-color STORM images are shown in Figures 2 d f.
Using Alexa 750 in conjunction with Alexa 647, we obtained two-color STORM images with multiple emission channels.
Hence, a detection setup using two non-overlapping emission channels is suitable for two-color STORM imaging with these dyes.
(E and F ) 2-color STORM and quantification of agrinC and podocaylxin labeling along a capillary region.
Since Alexa-647 also blinks in this buffer, it can be used for two-color STORM imaging.
For two-color STORM imaging experiments, we added an additional 405 nm laser (100 mW Coherent Cube), via an optical fiber.
Therefore, a 20%Vectashield-80Vectashield-80l mixture makes an adequate buffer for two-color STORM imaging using Alexa-555 and Alexa-647.
For two-color STORM imaging, abNup358 was labeled with both Cy3 and Cy5 (in an approx. 4 1 ratio), and importin-β was labeled with Alexa405 and Cy5.
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