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To verify that Obol-expressing cells were truely PGCs, we performed double color FISH to see whether Obol-positive cells were positive also for Odazl and Olvas.
Eleven cases that presented adequate tumor samples on slides were evaluated by dual color FISH.
We then performed a dual color FISH experiment with the α-satellite probe and CH271-340F4 on HLE chromosomes.
Dual color FISH experiments were performed to independently map each of the chromosome breakpoints (Fig. 3; Table 1).
Double color FISH was performed as described [ 15] using chr3 specific painting probe labeled with FITC (Cambio, Cambridge, UK), combined with biotin labeled RP6-123i13.
Dual color FISH using differently labeled control probes was performed, and signal screening was carried out on at least 200 cells with well-delineated signals.
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Fluorescence in situ hybridization (FISH) was performed on mitotic chromosome spreads [ 51] and dual-color FISH analysis was carried out using 5S rDNA and 18S rDNA probes.
d Two-color FISH assay results.
These results confirm the applicability of the developed permeabilization protocol, combined with the double-color FISH technique in specific detection of B. anthracis in soil and air samples.
The results have proven the success of double-color FISH in detecting B. anthracis spores in air samples in the range of 103 spores/m3 and above.
The effectiveness of the two-color FISH assay depends on the physical proximity of two linked loci within the nucleus.
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