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The identity of the incorporated nucleotide was determined by serial colorimetric reactions with anti-FITC-alkaline phosphatase and streptavidin-horse radish peroxidase, resulting blue and/or yellow color developments were analyzed with an ELISA reader and the final genotyping (allele) calls were made with the QCReview program.
The average well color developments (AWCDs) of the different replicates were calculated, where AWCD equals the sum of the difference between the OD of the blank well (water) and substrate wells divided by 95 (the number of substrate wells in the FF plates) developed by the fungus after 216 h of incubation.
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average well color development.
Freshly prepared DAB was added for color development.
Hybridization signals were read by NBT/BCIP color development.
where P i is the proportional color development of the ith well over the total color development of all wells in a plate.
The Comet cleanser seems to mask the p-dimethylaminobenzaldehyde color development".
A biotinylated HRP system was used for color development.
Color development was determined by measuring absorbance at 450 nm.
Color development was stopped using 1 M phosphoric acid.
The substrate used for color development was tetramethylbenzidine.
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