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Hybridization signals were read by NBT/BCIP color development.
For color development 3,3′-diaminobenzidine tetrahydrochloride (Sigma) was employed and hematoxylin was used as counterstain.
Finally, color development was performed with diaminobenzidine (Dako Denmark A/S) as the substrate.
average well color development.
Freshly prepared DAB was added for color development.
where P i is the proportional color development of the ith well over the total color development of all wells in a plate.
The Comet cleanser seems to mask the p-dimethylaminobenzaldehyde color development".
The substrate used for color development was tetramethylbenzidine.
Color development was stopped using 1 N H2SO4.
The color development was carried out for 6 7 minutes.
A biotinylated HRP system was used for color development.
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