One particular piece called, Chromosaturation, located in the museum's courtyard, features a series of connecting color chambers that are housed within a large shipping container.
One particular piece called, Chromosaturation, located in the museum's courtyard, features a series of connecting color chambers that are housed within a large shipping container.
For two-color imaging, chambers were washed three times with imaging buffer (supplemented with a GLOX system to minimize photobleaching), followed by 5 min incubation with neutravadin.
"Creating the head was a lenthy process involving casting, moulding, and layering color into the chamber, not to mention the stop-motion heating process used to capture the sequence," he explains.
To further distinguish the boxes, a unique odor was placed into a wall-mounted plastic container matching the color of each chamber.
The recording cage was placed in a well-ventilated, sound and light dampened (black color plexiglass) recording chamber (48′′ × 24′′ × 24′′) to minimize external disturbances during the experiments.
For single color experiments, the chamber was washed three times with imaging buffer (BRB80 pH 7.2 supplemented with 100 mM KCl, 0.5% BSA, 0.5% methylcellulose, and 40 mM βme). 4 mM GTP was added to 2.5 μM Cy3-PhuZ (80% wild-type, 20% Cy3-labeled) in imaging buffer, flowed into the chamber, and subsequently imaged.
Apical 5-chamber color Doppler evaluation of the LVOT and aortic valve revealed severe aortic stenosis (Fig. 4 and see Video Clip S3 available as supporting information in the online version of this paper).
In his scoring Schoenberg tries to enhance and project every harmonic nuance and instrumental color in the original chamber work.
He may be a white male, but the new Republican U.S. Senate hopeful from Oklahoma could bring some much-needed color to the upper chamber -- at least as far as hair follicles are concerned.
