This method demonstrates a combination of off-the-shelf inkjet printing and biomaterials and has potential to be adapted to tissue engineering and colony patterning applications.
The number of CFUs was evaluated and isolates were classified as "none", "1-10 colonies", "dense, but separate" and "confluent" colony patterns according to the density of the culture.
After the reisolation of P. multocida from liver and cardiac blood of infected animals, colony patterns were evaluated (Table 1, Figure 2).
A considerable part of computational efforts has been devoted to the simulation of branched colony patterns, which various microbes are known to produce under harsh growth conditions [ 12, 15, 18, 19].
When transformants of control plasmids pQ0 or pQ30 were plated on 3%% glycerol medium, growth was generally slower compared to glucose plates, but the colony patterns after four additional days of incubation resembled those of yeasts grown on glucose (Fig. 7a).
Images of fluorescent colonies patterned on agar plates were acquired using a Typhoon 9400 fluorescent scanner (GE Amersham Biosciences).
Although the effects of habitat heterogeneity on the within-colony patterns of distribution in birds have long been hypothesised, most of the empirical evidence has only been circumstantial and the suggested relationship has never been supported by a formal analysis.
Epistem-like B9-2-5 was cultured in typical non-mouse ESC/iPSC media containing bFGF (Fujishiro et al., 2013), and its colony shape and gene expression patterns were similar to those of mouse epiblast stem cells (EpiSCs; Brons et al., 2007; Tesar et al., 2007).
Recently, pluripotent cells were divided into two categories: naïve and primed, distinguished by colony morphology, pattern of gene expression and other features [ 33, 34].
