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All patients underwent a colonic preparation using a low-fibre diet, 2 l polyethylene glycol electrolyte solution (Moviprep; Norgine, Mid Glamorgan, UK) and faecal tagging.
Introduction: Colonoscopy is crucial for the management of lower gastro-intestinal disorders, but its profitability is discussed in critically ill patients, mainly because of the complexity of colonic preparation.
In infants with S-type HD, preoperative colonic preparation is usually manageable using a glycerin enema, serial rectal decompression, or washouts, whereas most infants with longer type aganglionosis require diverting stoma (colostomy or enterostomy) followed by staged surgeries to complete curative treatment.
Additional exclusion criteria were an unsatisfactory colonic preparation ("poor", see below) precluding a complete examination.
Sodium phosphate (NaP), a buffered saline laxative, gained popularity as an alternative method for colonic preparation due to its smaller volume.
The quality of bowel preparation was not formally reported but only six of 1253 patients were excluded initially because of inadequate colonic preparation.
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The application of gastrin-17 to colonic preparations resulted in relaxant responses which were insensitive to devazepide, and prevented by GV150013, l-NAME or tetrodotoxin.
The application of cholecystokinin octapeptide sulphate (cholecystokinin-8S) to colonic preparations induced concentration-dependent contractions which were prevented by devazepide (CCK1 receptor antagonist), enhanced by GV150013 (CCK2 receptor antagonist) or Nω-nitro-l-arginine methylester (l-NAME, nitric oxide synthase inhibitor), and unaffected by tetrodotoxin.
Next, we investigated whether SF3B-containing diet intake affects the restoration of enteric neurotransmissions being damaged during induction of colitis by TNBS using isolated colonic preparations.
Early studies showed that tegaserod facilitates the peristaltic reflex in vitro in human small intestine, and guinea pig and rat colonic preparations.
An initial tension of 1.0 g was applied to the colonic preparations, which were subsequently allowed to equilibrate for 30 min.
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