Ductile lower crustal flow affects not only orogenic belts in collision settings but also rifted passive margins.
Although the accretion of magmatic arcs is considered a primary mechanism by which the continents have grown over most of Earth's history (Hamilton 1981; Card 1990), the tectonic processes responsible, such as accretionary wedge development and obduction/underplating of the arc crust, are poorly documented, especially in arc-arc collision settings.
The kit came equipped with, MRM transitions for analytes of forensics and toxicological nature along with their optimized fragmentor voltages and collision energy settings.
Doubly and triply charged peptide-ions were automatically selected and fragmented with m/z-dependent collision energy settings.
To this end, the detector was run in daughter ion scanning mode and the cone voltage and collision cell settings were optimized to obtain as many fragments of the respective compound as possible.
Figure 1A shows that for all tested HCD collision energy settings, the percentage of HCD spectra where one or more reporter ions failed to be detected was strongly reduced with the HCD collision cell with an axial field (paired t test p < 0.0001).
To confirm that optimal conditions were achieved for the HCD analyses under the reduced pressures required for online intact protein and product ion detection, the samples were run using three different normalized collision energy settings (NCE 15, 25, and 35) and the results compared (Supplemental Figure 3 in the Supporting Information).
Moreover, the instrument was operated on the following settings: collision energy of 3 eV, capillary voltage of 2.5 kV, sample cone voltage of 30 V, detector voltage of 1650 V (1600 V in negative mode), source temperature at 120 °C, cone gas flow at 50 (L/h), and desolvation gas flow at 550 (L/h).
Tandem MS (MS/MS) analyses were carried out with identical ESI parameters, and the following fragmentation and precursor ion selection settings: collision energy 10 V, precursor isolation window 1.3 amu, and scan rate 1.00 spectra/s.
cell acceleration voltage aValues are given in the order quantifier ion, qualifier ion (in parentheses are the corresponding collision energy (CE) settings in volts) bDefined as the peak area of qualifier in per cent of the quantifier Chromatographic separation was performed by reversed phase chromatography using acidified water and methanol as mobile phases.
In collision athletes or revision settings with possible poor quality of the CA ligament, we do consider the use of autograft or allograft supplementation.
