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Higher energy collision dissociation.

It also enables the popular electron-transfer dissociation, which uses a charged donor molecule to induce fragmentation, as well as hybrid methods, such as electron-transfer and higher-energy collision dissociation (EThcD).

Different fragmentation methods including collision-induced dissociation (CID), higher-energy collisional dissociation (HCD), electron-transfer dissociation (ETD), and electron-transfer/higher-energy collision dissociation (EThcD) (Liu et al. 2014) have been used to varying degrees of success.

As shown in Fig. 7, we found that short reaction times of 10 ms together with 40%% supplemental higher energy collision dissociation (HCD) activation gave better results than longer reaction times and lower or higher activation energies.

Here, we demonstrate a novel electron-transfer/higher-energy collision dissociation (EThcD) approach that preserves original reporter ion channels, mitigates bias against lower charge states, improves sensitivity, and significantly improves data quality for quantitative proteomics and proteome-wide PTM studies.

The Q Exactive mass spectrometer was operated in the data-dependent acquisition mode using the Xcalibur 2.1.3 software and there was a single full-scan mass spectrum in the Orbitrap (400 1800 m/z, 30,000 resolution) followed by 10 MS/MS scans in the quadrupole collision cell using the higher energy collision dissociation.

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Similar(14)

Ions were fragmented using collision-induced dissociation (collision energy 35%, 30 ms).

Argon served as the collision-dissociation gas (0.18 L/min flow rate), and nitrogen was the desolvation gas (500 mL/min flow rate) [ 15].

The high-resolution and collision-dissociation FT techniques described above were used in this work to separate and identify two by-products of the polymerization between 12-hydroxystearic acid (HSA) and stearic acid (SA), to provide precise information about the exact location of caprolactones on the Tris 2-hydroxyethyl)isocyanuraTris 2-hydroxyethyl, and to sequence a GMA/MMA copolymer.

MS conditions were as followed: spray voltage, 1.8 kV; heated capillary temperature, 150°C; normalized collision-induced dissociation (CID) collision energy 37.5% for MS/MS in LTQ.

Every selected precursor was fragmented by collision induced dissociation (CID) and electron transfer dissociation (ETD), respectively.

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