A high stringency (P ≤ 0.0001) screen of all neointron sequences of D. pulex using RepFind (Betley et al. 2002) identified 312 significant repetitive motifs shared among neointrons ranging in length from 4 to 17 bp and in frequency from 2 to 304 occurrences in the collective set of all neointron sequences (supplementary table S4, Supplementary Material online).
First, a high stringency screen (P ≤ 0.0001 in RepFind) of exons, which are the host of neointrons, revealed 130 repetitive motifs ranging in length from 4 to 15 bp and in frequency from 2 to 172 occurrences in the collective set of all such exon sequences (supplementary table S5, Supplementary Material online).
We and others have demonstrated that, likely owing to reductions in β1-integrin protein, knockdown of JAM-A gene expression in epithelial cells results in decreased collective cell migration [ 19, 27, 28] (Supplementary Figure S1A in Additional file 1).
First, it would be desirable to measure the actual replacement rates better by taking account of supplementary benefits provided by unions or in collective agreements.
In all cases it is apparent that the computed displacements are significantly greater than the changes in the internal structure of these elements, quantified in Supplementary file 1 and Figure 2 figure supplement 1, indicating collective motions take place.
22 The HWE p value for all cohorts individually was >0.3 with the exception of LBC1921 (p=0.08), while the collective p value for all cohorts combined was 0.73 (see online supplementary table S3).
While this represented a very different experimental paradigm and gene expression studies were performed following 12 and 48 h of RAD001 oral administration, a collective analysis showed a similar mTORC1-dependent induction of V-ATPase expression (Supplementary Figure S5).
Blebbistatin treatment decreased the speed at which ring closure occurred and inhibited collective cell motility, although single cells were motile and exchanged position with neighbours (Supplementary Video 2).
Formalin-fixed paraffin-embedded tumor tissues, obtained from a collective of 60 GBM patients, were analyzed for the expression of miR-125a/b (Supplementary Figure S2).
