The block was trimmed and collection of sections occurred approximately 2 mm on both sides of the site of the injection.
Collection of sections onto gelatin-coated slides commenced rostral to the anterior nuclei of the BST (bregma + 0.2 mm, Paxinos and Watson, 1998) and continued to a level that was caudal to the central amygdala (bregma −3.3 mm).
Collections of cryostat sections prepared from the tissue samples were evaluated by a pathologist to control the relative cell type content.
Then, collections of cryostat sections were prepared from different parts of each tumour fragment.
Thick samples for tomography (200 300 nm) were either floated onto 200 mesh square Ni grids or onto formvar film coated Cu 1×2 mm slot grids (Agar Scientific Ltd., Stansted, UK) to allow for the collection of serial sections.
Before the investigation, the entire collection of brain sections, was subjected to a neuropathological whole-brain analysis for clinical diagnostic purposes, according to routine procedures at the Department of Pathology, Division of Neuropathology, Lund University Hospital.
Breast cancer cells showed nuclear, cytoplasmic or mixed maspin location.In order to analyze the subcellular location and assess the level of expression of maspin in normal and tumor tissues of breast, a collection of tissue sections from 166 patients with invasive ductal breast cancer was studied using immunohistochemistry.
Two collections of whole tissue sections of breast carcinoma were immunostained for biomarkers.
In further support of this alternative position on NB that questions their proposed involvement in the development of kidney stones via Randall's plaque, it should be pointed out that Ryall's review [29] contains an impressive collection of thin section TEM images of the lamellar granules comprising Randall's plaque (see her Fig. 10).
As far as we know, this paper treats the largest collection of Solanum section Petota accessions ever analysed simultaneously.
