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Table S6 provides a summary of all mutant allele collections for analysis of essential genes in yeast.
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All patients enrolled in the study had a baseline blood sample collection for analysis of circulating nucleic acid for mutations in PIK3CA.
Blood was drawn in 5-mL collectubestubes for analysis of serum testosterone, cholesterol, and triglycerides.
Cells were incubated for 5h before the collection of supernatants for analysis of IL-1β release.
A 9-month follow-up interval was chosen for collection of blood for analysis of novel risk factors.
At the last weighing, a group faecal collection was made for analysis of copper content.
The cells were then treated with inhibitors as indicated for 15min, prior to addition of the sphingolipid metabolites at the indicated concentrations for 1 h and collection of supernatants for analysis of IL-1β processing and release.
This involved collection of urine samples for analysis of 1-hydroxypyrene and collection of exhaled breath condensate for measurement of pH and hydrogen peroxide.
The patients' sputum was collected by spontaneous sputum sampling, induction by physiotherapy maneuvers, or collection of oropharyngeal swabs for analysis of the microorganism colonizing or infecting the airways.
A 1 mL aliquot of each sample was placed in a Quantisal collection tube and sent for analysis of Δ9-THC content using LC/MSMS by Synergy Health.
The collection of patient samples for analysis of genetic and epigenetic changes was approved by the local ethics committee.
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