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Before spectrum collection, the sample was first thermally pretreated at 350°C for 1 h under high-purity N2, then another 1 h under high-vacuum condition.
The differences in timing of data collection, the sample sizes and the underlying populations between the two data sources suggest that neither of these data sets should be considered preferable, a-priori.
The load feedback function in the 250 t press is less favorable during high-temperature data collection; the sample height may change because of the uniaxial nature of the opposed anvil devices, as the hydraulic ram compresses the HPXMT module along the vertical axis.
Immediately after each effluent collection, the sample was weighed.
To minimize the potential losses during data collection, the sample was increased by 20% (n = 720).
Immediately after faecal sample collection, the sample was frozen at −20°C.
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Upon collection, the samples were kept frozen at −20 °C.
Following the NMR data collection, the samples were examined with electrospray ionization mass spectrometry to provide additional structural information.
Immediately after collection, the samples were centrifuged at 4 °C and 10,000g for 20 min.
After collection, the samples were centrifuged, and the plasma was obtained for determination of hormones and interleukins.
After collection the samples were stored at −20°C.
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