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Preeclampsia was defined as severe if (1) the systolic blood pressure was > 160 mmHg or the diastolic blood pressure was > 110 mmHg on at least two occasions after 20 weeks of gestation, or (2) there was ≥ 5 g of protein in a 24-hour urine collection specimen or ≥ 3+ proteinuria on dipstick urinalysis of two samples collected at least 4 hours apart.
Details regarding specimen collection, specimen processing, and genotyping were previously reported [ 44].
In patients with renal involvement, the extent of proteinuria was determined by measuring total protein in a 24-h urine collection specimen.
Therefore, we have not shown that the failure of the long range PCR was caused by the fact that we used an 'aged' collection specimen.
Information was extracted by one reviewer (JC) on: type of study, year of data collection, specimen type, diagnostic test, age group and gender of participants, response rate, number of individuals tested, and results.
Although a more extensive evaluation of capillary blood specimens under field conditions is still needed, our results point toward the use of capillary blood samples as an appropriate collection specimen for sensitive and specific diagnosis of EVD.
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After collection, specimens were immediately transported to the laboratory for inoculation onto culture medium.
After collection, specimens were snap-frozen in liquid nitrogen and stored at −80 °C.
Upon collection, specimens were euthanized in Eugenol and fixed in 10% formalin.
Within 30 min of the sample collection, specimens will be transferred to a laboratory.
Culture collection specimens entered in the strain or isolate fields were moved to the culture collection field.
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