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For collection of milk samples, sows were given 20 I.U.
This study entailed collection of milk samples from farmers' cattle.
After blood collection, dams were given one unit oxytocin to stimulate lactation approximately 5 min before collection of milk samples.
Thanks to Michael O'Flaherty for facilitating the collection of milk samples at his farm, to Margaret Murray for assistance with milk sample processing.
The authors would like to acknowledge: the Foundation for Research Levy on Agricultural Products for funding the project; the farmers involved for their careful recordings and patient collection of milk samples; Ellen Dahl and Kjersti Finsrud for their assistance in analysing the samples; the late Kjell Andersen Berg for his contribution toward the collection of field data.
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Given that S. aureus and CNS were the pathogens isolated most frequently from cases of subclinical mastitis, one possible explanation for resistance to several antibiotics may be the collection and submission to the laboratory of milk samples from chronic clinical mastitis (which demonstrate poor treatment efficacy).
Data collection was done through key informant interviews, structured interview schedules, observation and microbial analysis of milk samples.
Over 60% of milk samples tested positive for antibiotic in Nigerian milk and other milk products (Olatoye et al. 2016).
No difference existed in the timing of collection of the milk samples between the two populations (Table 1).
To further complicate correlations among analytes, the timing of milk sample collection was not uniform among donors or among days of individuals.
The collection of breast milk samples in our study covered a 5-year period, and we found a negative correlation between the level of PBDEs in breast milk and the infant date of birth.
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