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Here we present a collection of medium and large-scale dynamic systems, with sizes of tens to hundreds of variables and hundreds to thousands of estimated parameters, which can be used as benchmarks for reverse-engineering techniques.
In addition, the systems would be amenable to the collection of medium samples or the harvest of cellular material for subsequent biochemical and metabolic analyses during or after completing experimental protocols.
After collection of medium, the remaining cells were lysed in 0.9% (w/v) Triton X-100, and LDH content in medium and lysed cells was measured to determine total LDH content.
In British Columbia there are two large metropolitan centres (Vancouver and Victoria) located in the southern part of the province, and a collection of medium to small towns with low density population distributed across the interior, the northern part of the province, and Vancouver Island.
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BBC executives are understood to have concluded that a national digital radio station remains the best way to reach Asian listeners, rather than a collection of medium-wave services that was mooted as one possible replacement.
Ireland's collection of medium-pacers and spinners may have been able to use the conditions to their advantage, but regular width aided the Pakistan cause.
By combining the proposed deep CNN and the huge collection of medium-resolution remote sensing data, we believe that much more accurate land cover datasets can be produced over large areas.
Featuring nearly 70 different artworks by more than 40 artists, including William Eggleston and Andy Warhol, Shrines to Speed is a collection of mediums; photographs, installations, and paintings are all present.
In the nexus of entertainment and information that includes what we traditionally have thought of as television and radio and that which we call the Internet — a collection of mediums that have begun to blend together as they reach us increasingly across the same platforms — one modality rules over all: talk.
For the collection of medium-speed membrane pellet, Atg5 KO MEFs and WT MEFs were cultured, collected and lysed in B88 buffer as described previously (Ge et al., 2013).
Cells were seeded in 48-well culture plates at a density of 3 × 10/well and incubated for 24 h before the medium was replaced with serum-free medium (Invitrogen, Carlsbad, CA), followed by collection of conditioned medium and quantification for protein contents.
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