Sentence examples for collection of conditioned from inspiring English sources

For collection of conditioned media, cell lines were grown to 80% confluence and washed with PBS and then cultured for 24 hrs in their respective serum-free media.

macrophage cultures were washed three times to remove noningested material and further cultured in serum-free medium (GIBCO 31885) for 24 h prior to collection of conditioned media.

Vehicle- or TNFα-treated cultures were discarded after the collection of conditioned media.

For the collection of conditioned media, cells were treated for 24 hours before harvest and stored at −20°C.

Following collection of conditioned media, cells from different wells were trypsinized and counted to normalize the MMP-2 secreted.

Briefly, 4×10 cells were seeded in phenol red-free DMEM/F12 medium supplemented with charcoal-stripped serum (5%) and adequate growth factors under normal conditions for 72 hrs before collection of conditioned medium.

Observe from the above application of Corollary 3.3 that we have a sort of unusual collection of conditions - namely, that y must be 'initially' negative, increasing, and convex.

In other words, the collection of conditions (f(a)le0), (Delta f(a ge0), (Delta_{a}^{mu}f(t ge0) is not sufficient to deduce that (Delta^{2}y(t)ge0) holds for (tinmathbb{N}_{a+1}).

The work in this section supplies a variety of conditions on the relative positions of various trajectories such that when a set of inputs allows an appropriate collection of conditions to be satisfied, a pocket or rostral rhythm results.

Amyloidosis refers to a collection of conditions in which abnormal protein folding results in insoluble fibril deposition in tissues.

The autism spectrum disorders are a collection of conditions, which have, in common, impaired socialization and communication in association with stereotypic behaviors.