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This entire collection of alignments contained a total of 5.9 million introns.
Clustering allowed a given TIC exon-exon junction to be supported by the entire collection of alignments in demonstrating a sufficient overhang.
YAHA's OQC algorithm is designed to select the optimal collection of alignments and should handle such situations better than heuristic strategies.
The likelihood of each site is calculated given the particular ST and combined across the alignment or collection of alignments; in the latter case, all sites across all alignments are assumed to have evolved along precisely the same tree.
Unfortunately, experimental data is rarely available in sufficient amount, and when using several packages, one is usually left with a collection of alignments whose respective value is hard to assess in absolute terms.
A significant similarity of this region was established by running HHPRED against a collection of alignments assembled from Pfam and other domain alignments (KH, unpublished) (Soding et al, 2005; Punta et al, 2012).
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We believe that this OQC calculation allows for the discovery of biologically meaningful collections of alignments.
YAHA's use of an optimal DAG algorithm for discovery of the OCS and its ability to find collections of alignments similar to the OCS are completely novel.
With a relatively high maxGDP, collections of alignments near each other on a reference chromosome will be favored, helping to identify deletions, tandem duplications and inversions.
The inclusion of a genomic distance penalty in the objective function of YAHA's OQC algorithm undoubtedly aids its performance in these tests, as it allows YAHA to favor collections of alignments for the OCS that are near each other in the genome.
It works as follows [ 9, 6]: for a fixed alignment of sequences, a collection of new alignments is generated by deleting some columns from the alignment at random and replacing them by other columns from that alignment, also picked at random.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com