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After collimating the SHG beam with the collection objective, both objectives remain immobile for the rest of the experiment to maintain the collimation.
A 0.75 NA, 40x collection objective with a 4 μm depth of field was used and data were collected using INSPIRE 4.0 Software.
By using a collection objective with a numerical aperture higher than the focusing one, efficient detection of the forward SHG light is ensured.
With this setup, the radial distribution of the intensity in the collimated beam of SHG leaving the collection objective corresponds to the angular intensity distribution of the signal generated by the sample (also called, the radiation pattern).
The XYZ position of the collection objective was carefully adjusted with a micrometric positioner to ensure a collimated beam propagating along the same path as it would if the two objectives were absent.
Despite the fact that varying the collection objective's effective NA with an iris might look like a rudimentary setup, the next experiment demonstrates its validity for detecting changes in the distribution of noncentrosymmetric structures within the focal volume.
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Vessel Monitoring Plan (VMP) means the document that describes how fishing operations on the vessel will be conducted and how the EM system and associated equipment will be configured to meet the data collection objectives and purpose of the EM program.
We trained all of the assessors on the data collection objectives, procedures and tools, the consent process, and ethical issues.
Prior to the start of data collection, study personnel visited each health facility in-charge to brief him or her on data collection objectives and coordinate data collection on the days when antenatal and postnatal services were provided (Table 3).
At each health center, study personnel visited the health center in-charge to brief him or her on data collection objectives and ascertain the days antenatal and postnatal services were provided to coordinate timing for data collection.
The focus group was successful in identifying the key issues with respect to all the data collection objectives, resulting in a significantly shorter and more topically focused survey questionnaire.
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