Sentence examples for collection buffer from inspiring English sources

Study Design: Genomic deoxyribonucleic acid and total ribonucleic acid were extracted from cell pellets obtained from the residual fluid-based Papanicolaou specimen collection buffer after clinical processing.

The sampled peritoneal lavage fluid was centrifuged at 400×g for 5 min and then washed with the collection buffer.

Cells were washed twice in ice cold PBS and suspended in collection buffer (100 mM Tris-HCl pH 9.4, DTT 10 mM Protease inhibitors).

Cells were washed two times with PBS and resuspended in 1 ml of collection buffer.

A surgical laparotomy was performed, and the uterine horn was removed and placed into ice-cold tissue collection buffer.

Total protein extracts were quantified using the Bradford assay and diluted in collection buffer (1×CHAPS) to concentrations of 50 μg/ml, 10 μg/ml, and 2 μg/ml for incubation with the telomere repeat DNA template.

After collection, the buffer capacity of the saliva samples was determined using the CRT®buffer test (Ivoclar Vivadent, Schaan, Lichtenstein) as follows: the entire reaction pad was wetted with saliva.

For glucose-stimulated insulin secretion, cells were incubated in HBSS for an additional 2 hours in the presence of 3 mM, 15 mM glucose or 15 mM glucose+30 mMKCl as indicated followed by collection of buffer for insulin radioimmunoassay (Coat-A-Count kit, DPC).

Following cell collection, lysis buffer volume was increased to 100  μL, and the sample homogenized by vortexing and then placed at −80°C for later use.

Therefore, in order to assess the influence of time from swabbing to extraction on DNA yield and purity, buccal swab samples from the same person (n = 4) were collected and stored for 2 weeks, for 1 week, for 1 day and for a few hours in the collection medium (buffer and enzyme) before DNA was isolated.

Therefore, the number of frames to be searched of the collection and the Buffer window size are now shown in (12) and (13), respectively, (12).