Exact(1)
Although a hydroponic culture system does not reflect natural growth conditions for plants compared with a soil experiment, it is widely used (Gahoonia et al. 2000; Dechassa and Schenk 2004; Ligaba et al. 2004; Wang et al. 2013; Cheng et al. 2014) and useful for studying root architecture, including tiny root tips, and for collecting root exudates for biochemical analysis of OAs.
Similar(59)
We might imagine them not braving mammoth and bison, but collecting roots, shellfish and seaweed, and tracking grazing animals such as deer coming down to the water to drink.
In the two trips that followed, emphasis was placed on collecting roots of seedlings and mature plants alike.
They identified affected areas in aerial photographs and collected root samples from 112 dead and dying trees, mostly firs.
All collected root tips in the four sampled forests were sequenced (593 root tips).
Moreover, it is difficult to collect root surface washings in a system without causing physical damage to the roots, which is unlikely to occur in the artificial soil system.
The collected root was air dried and powdered.
The collected root exudates were immediately frozen at −20 °C.
The collected root exudates were immediately frozen at -30°C, freeze dried, and weighed.
Finally, the root mass was carefully immersed in a 1-L dark bottle containing trap solution for 24 h to collect root exudates.
Finally, cycle threshold (Ct) values (n = 4) were implemented in the standard curves' equations to quantify the amount of Pythium DNA in the collected root samples.
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