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The cell lysate is directed to cell lysate collecting chambers.
The cell lysates were maintained in cell lysate collecting chambers through the through-hole at the bottom of each microwell.
f The cell lysates are separately collected from cell lysate collecting chambers for the following DNA amplification and sequencing.
For cell lysate collecting chambers, through-holes were fabricated by piercing the PDMS layer (with needles) to form the lysate outlets.
As shown in Fig. 1a, from the functional point of view, the microfluidic chip consisted of three layers: the microfluidic channel, the cell trapping array and the cell lysate collecting chambers.
To lyse all trapped cells, after opening all bottom outlets and closing top outlet, cell lysis solution was pumped into the channel to fill cell trapping array and all cell lysate collecting chambers (schemed in Fig. 1e).
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Far too many interests for someone trying to have a career: board games, Dungeons and Dragons, astronomy, stamp collecting, chamber music.
Click HERE to view full-size graphic The study, published in the journal Nature, shows that graphene and a similar single-atom-thick material called boron nitride allowed the build-up of protons on one side of a membrane, yet prevented anything else from crossing over into a collecting chamber.
Each lysate collecting chamber covered 100 cell trapping chambers.
Heavy and light phase collecting chamber are set up between drum and lining.
Both the microfluidic channel and cell lysate collecting chamber were fabricated by PDMS.
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