The N T1 data were collected with relaxation delays of 10, 110, 190, 320, 400, 520, 630, and 750 ms. The N T2 data were collected with relaxation delays of 8, 32, 64, 96, 128, 192, and 248 ms. The N heteronuclear NOE data sets were acquired with the unenhanced and enhanced spectra interleaved and a 3 s proton excitation period for the NOE-enhanced spectrum.
For the 2D correlation NMR spectra, complex data points of 1024 × 290 were collected with an acquisition delay of 3 sec.
The spread of an infectious disease can be strongly influenced by behavioral changes (e.g., social distancing) during the early phase of an epidemic, but data on risk perception and behavioral response to a novel virus is usually collected with a substantial delay or after an epidemic has run its course.
N Heteronuclear NOE (nuclear Overhauser effect) experiments were collected with a relaxation delay of 5 s with and without saturation of the amide protons that was achieved using 120° high-power pulses [ 16].
All LIBS spectra were collected with a time-delay of 1.3 μs with respect to the laser pulse and for an integration time of 1 ms.
N T2 data were collected with 10 different relaxation delays of 20, 40, 60, 80, 100, 120, 140 and 160 ms including repeats for 80 and 160 ms delays.
Spectra were collected with a 1.5 s repetition delay, 64 steady-state transits, 2 acquisition transits, 128 increments, and an acquisition time of 0.5 s (7530 points).
Spectra were collected with 2 k data points, 0.5 s delay times, and averaged over 45,000 scans.
For the R1 and R2 measurements, 512 (1H) and 128 (15N) complex data points were collected with 32 transients per increment and a recycle delay of 2.5 s.
Spectral widths of 11160.7 Hz for 1H and 2432.8 Hz for 15N were used, and 512 (1H) and 128 (15N) complex data points were collected with 32 transients per increment and a recycle delay of 2.7 s.
These qualitative conclusions were independent of the surrogate receptor, as data collected with DC-SIGN-R had only slightly shorter delay times and slightly higher hemifusion rates.
