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Data were retrospectively collected which may have resulted in an underestimation of toxicity occurrence.
In addition, data on the resolution of acne were not collected, which may have impacted compliance data.
The selection bias cannot be avoided because of the relatively small number of samples collected, which may reduce the representativeness of our results and capacity for inference.
The final limitation concerns the fact that only behavioural data were collected which may not reflect the respondents' actual risk for HIV or STIs.
First, data were retrospectively collected which may have led to potential bias in the evaluation and characterization of the etiology of ARF among centers as well as the collection of information on do-not-intubate orders.
Thus, in addition to the real differences in species richness different raw species richness values may occur solely because different number of individuals have been collected, which may in turn reflect important differences in, for instance, resource availability or environmental conditions, but also differences in sampling effort or conditions in sampling [ 42].
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Data on several characteristics of male subjects, e.g., jobs, lifestyle, and educational levels, were not collected, which potentially may be related to compliance with therapy in the current study.
Now at the end of five years, only 17 cases of primary malignant bone tumors have been collected which in our opinion may be considered cured (Ewing's tumor, 4 cases osteogenic sarcoma, 13 cases).
Existing CPRs for assessing cancer risk have often been developed in secondary care populations, 9 meaning they may be less accurate in primary care, and others 10, 11 have been derived using only routinely collected data, which may not provide valid, standardised information in the way that a study designed specifically to collect information on diagnostic indicators would do.
Therefore, the carbon stocks estimated in this study carry large uncertainties, including from the approach of adopting a statistical mean to represent the collected data, which may artificially bias calculated values.
The data on exposure variables were collected retrospectively, which may have introduced recall bias.
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