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Specimens collected were maintained in a cooler containing ice blocks.
Tallies of data collected were maintained at the project headquarters so that under-sampled individuals could be given top priority for data collection.
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The mechanism, which has been in place for only a couple of years, "was put into place to ensure the balance between the cost to provide electricity and the actual amount of revenue collected is maintained," said Garry Brown, the commission chairman.
Record of user-fee collected was maintained by the medical officer in-charge.
The quality of data collected was maintained through the use of standardized protocols and centralized training.
The collected cells were maintained in DMEM containing 20% FBS and penicillin/streptomycin.
Epitympanic taps were attempted two and four days after bacterial challenge and collected fluids were maintained on ice until serially diluted and cultured on chocolate II agar plates (Fisher, Pittsburgh, PA).
Collected blastocysts were maintained in culture for one day in M16 medium to reduce contamination by maternal tissues, and then lysed to purify genomic DNA for analysis.
Collected termites were maintained under laboratory conditions (22°C with 70% relative humidity and a 0 24 light:dark photoperiod) on wood shims and paper towels before the feeding assays.
Finally, the collected implants were maintained intraorally for only 2 months in this study, and further long-term study is required to confirm the existence of osseohybridization zone in long-lasting implants.
After these observations, the collected trail ants were maintained singly without food and died within a few days without signs of O. unilateralis s.l.l
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