The iodide concentration of each aliquot collected was determined using an iodide-selective electrode (Thermo Scientific, Waltham, MA) and converted to iodide efflux rate (nanomol/min) as described previously.
Total C, N, H and S contents of collected samples were determined using a CHNS-O analyser (CE Instruments EA1110).
Cadmium (Cd), chromium (Cr), lead (Pb), manganese (Mn), copper (Cu) and iron (Fe) contents in collected milk samples were determined using Flame Atomic Absorption Spectrometry FAASS).
Proteins were collected and concentrations were determined using the BCA Protein Assay Kit Thermo Scientificc).
Lysates were centrifuged at 16,000 × g for 10 minutes at 4°C and the supernatants were collected; protein concentrations were determined using the bicinconinic acid protein assay (Pierce).
Whole cell lysates were collected, protein concentrations were determined using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and the protein concentrations were equalized.
Supernatants were collected, protein concentrations were determined using Bio-Rad protein assay, SDS sample buffer was added, and samples were boiled for 5 min and then frozen at –80 °C until used.
The supernatant was collected and protein concentrations were determined using BCA assay (Pierce, Rockland, IL).
The supernatants were collected and protein concentrations were determined using the Bradford method (Coomassie Protein Assay kit, Thermo scientific, Rockford, IL).
