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The supernatant collected was filtered into a clean sterile dried conical flask.
The supernatant collected was filtered with Millipore 0.22 μm syringe filters (Milford, MA, USA).
The medium collected was filtered through 0.2 mM filters (Millipore, USA) and stored, pooled, and concentrated using 3 kDa cut-off membranes filters (Millipore, USA).
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The samples collected were filtered through a membrane filter (0.45 μm) and further analyzed by HPLC.
The extracts collected were filtered and stored at -20°C.
Briefly, the media samples collected were filtered through 0.2 μm syringe filters.
Prior to analysis, the collected water was filtered using a standard 0.45 μm cellulose filter.
After distillation, the collected condensate was filtered and then prepared for determining nitrogen isotopic values.
The collected supernatant was filtered by means of a syringe connected to a 0.45-μm pore size filter.
The collected river water was filtered using a 0.22-μm polystyrene filter, to prevent microbial growth.
The water samples collected were first filtered (0.45??m) and then autoclaved to remove microorganisms in the waters.
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