To detect a prevalence of human pathogenic Y. enterocolitica of at least 3% with a 95% Confidence Interval (CI), the number of samples to be collected (n = 97) was calculated using the software program Win Episcope 2.0 [ 18].
The supernatant was collected, and the protein concentration was calculated using the Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL, USA).
Cells or tissues were lysed in RIPA lysis buffer (Beyotime, Shanghai, China) with freshly added PMSF (Beyotime, Shanghai, China) for 30 min on ice and were centrifuged at 12,000 × g at 4°C for 10 min. The supernatant was collected, and the protein concentration was calculated using a BCA protein assay kit (Thermo Scientific, Rockford, IL, USA).
The supernatant was collected, and the protein concentration was calculated using a Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL, USA).
Cell lysates or tissue homogenates were centrifuged for 10 min (12000 g, 4°C), the supernatant was collected, and the protein concentration was calculated using a Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL, USA).
The MELD score for the study cohort was calculated using labs collected before transplantation or, if unavailable, the last record of the wait-listed candidate was used.
Four 10-μl samples were collected from each probe, and RR was calculated using equation (3) with Cout defined as the mean MMC-concentration in the dialysates.
For RT-qPCR experiments, average RNA expression was calculated using data collected from three biological replicates and three technical replicates for each biological replicate.
