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These cold traps have collected volatile substances, including water ice, over geologic time.
The collected volatile compounds were identified using an Agilent gas chromatographer (GC) (6890N) coupled with a mass spectrometry (MS) system (5973 MSD, Agilent Technologies, Inc. USA).
(A ) (Left) The numbers of compounds present in the collected volatile library according to source.
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More recently we have collected volatiles using the SPME method as described here.
Collected volatiles were identified as described by Wei et al [19].
We also collected volatiles from four remaining undamaged vines and tested the undamaged vines in the flight tunnel.
First, we collected volatiles by using the rapid and solvent free Head-Space Solid Phase Micro-Extraction method (HS-SPME) initially developed by J. Pawliszyn [6].
After flushing the collected volatiles were desorbed from the Tenax traps at 220°C (Ultra; Markes, Llantrisant) for 5 min with a helium flow of 50 ml.min−1.min−1
He, however, collected volatiles 48 h after damage and focused on the emission of sesquiterpenes.
We collected volatiles for 16 h during photophase (06 00 22 00) and on a separate set of filters for 8 h during scotophase (22 00 06:00).
We collected volatiles during both the photophase and scotophase because previous studies documented substantial variation in volatile blends emitted during these phases and day- or night-active insects can be more responsive to the volatiles emitted during their times of peak activity [ 31, 33].
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