Sentence examples for collected treated with from inspiring English sources

Three days post-infection the cells were collected, treated with lysis buffer (25 mM Tris-HCl pH 7.4, 5 mM EDTA, 15 mM NaCl, and 0.1% NP40), and the lysate was centrifuged at 2,450 g for 30 min at 4°C.

Cells were trypsinized, collected, treated with trypan blue and the cells that excluded trypan blue (viable cells) were counted using a haemocytometer.

After 16 hr at 25° the embryos were collected, treated with 2% sodium hypochlorite, and washed with sterile water before RNA extraction.

The samples were centrifuged at 14,000 rpm for 10 minutes at 4°C and the supernatant was collected, treated with 100 μg/ml RNAse at 37°C for one hour.

To study the effect of cell density on transformation efficiency, cells of L. lactis LM0230 were collected, treated with LiAc and DDT, washed and diluted to various concentrations (Table 2).

To monitor the blood virus contents in virusemia phases (the 4thh and the 8thh) and stable (the 54thh) phases of the disease, blood samples randomly taken from 5 feathers per group at the 4thh, the 8thh and the 54thh after challenged with virus were collected, treated with heparin anticoagulation and used for testing its virus nucleic acid contents.

The supernatant was collected and treated with TRIzol LS for cytoplasmic RNA extraction.

The collected blood, treated with the anticoagulant K2-EDTA, was centrifuged at 5000 rpm for 10 min at 4 °C to separate the plasma.

Freshly-isolated surgical tumor specimens were collected and treated with collagenase for enzymatic dissociation.

At the end of incubations, cells were collected and treated with 50 µl RNase I (1 mg/ml) and stained with 5 ul propidium iodide (1 mg/ml).

For the determination of the serum levels of PGE2, the serum from mice were collected and treated with indomethacin (10 µg/ml).